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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might contribute to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants ended up documented but detailed nucleotide sequence facts just isn't yet readily available.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This guidebook outlines further doable optimization of mobile and immunoassay parameters to make sure the absolute best benefits are...
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This information outlines further more probable optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound website to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family members, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins exhibit unique expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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